DETERMINATION OF CONTENT OF HEAVY METALS IN HAIR WITH THE HELP OF METHODS OF DIFFERENTIAL PULSED POLAROGRAPHY AND X-RAY FLUORESCENT SPECTROSCOPY

Djaparidze Dj. I.¹, Shavgulidze N. V.¹, Havtasi N. S.¹, Enukidze L. G.¹, Harischarishvili I. Z.², Kirilenko E. K.³, Galchenko S. N.⁴

1. Institute of inorganic chemistry and electrochemistry named by R. I. Agladze, Tbilisi, Georgia.

2. Centre of medical elementology «Bioelement», Tbilisi, Georgia.

3. Scientific and technical centre «Viria», Kiev, Ukraine.

4. National Taras Shevchenko University of Kiev, Kiev, Ukraine.

This article deals with a topical problem of microelement analysis of biological substratums. Influence of toxic elements on organism was examined. We mostly drew attention to the following two methods of analytical analysis of bioassays: differential pulsed polarography and X-ray fluorescence.

The results of simultaneous determination of ions of Pb (II), Zn (II) and Cu (II) in hair were presented with the help of DPP method and of RFA. The comparative estimation of results from DPP and X-ray fluorescence method was conducted.

KEY WORDS: differential pulsed polarography, X-ray fluorescence, bioassay, microelement analysis

UDC 663.97:546:3:54.062

Roentgen fluorescent method of potable water microelement composition determination/

Galchenko S. N., Korotkov P. A., Kirilenko E. K.

The article is dedicated to the actual problem of potable water quality control. The main analytic methods of water quantitative analysis are considered. The express-technique for microelement water composition determination that is based on the roentgen fluorescent method is proposed. The concentration analysis results of 8 portable water samples are presented. Their evaluation is performed on the basis of the given maximum allowable concentrations.

Skin and nourishment./ S.V. Fus

The basic aspects of nutrient influence  on  complexion are considered. Sources of the basic nutrients which can provide daily maintenance for them are presented.

P. A. Korotkov, D. Sc., prof,. E. K. Kirilenko, Ph.D, S. N. Galchenko^*, postgraduate student.

 The practical use results of sensitivity increase technique in quantitative X-ray fluorescence analysis are presented. The technique is based on the method of maximum likelihood and can be used in the cases of the characteristic lines strong overlap and weak spectral lines in noise background. The spectra of mercury and zinc amalgam were analyzed, at first on the test samples of filter paper and than on the test bioassays of blood plasma solid. Absolute error of mercury content detection in bioassays was 2.4 times decreased. Detection limit achieved by the method is 13 ppb, significantly lower than obtained by standard spectral processing procedures.

SENSITIVITY INCREASE OF QUANTITATIVE X-RAY FLUORESCENCE ANALYSIS OF BIOASSAYS BY THE THERMAL RAPID CONCENTRATION METHOD

S. N. Galchenko, P. A. Korotkov, E. K. Kirilenko

The practical use results of sensitivity increase technique in quantitative X-ray fluorescence analysis are presented. The technique is based on the preconcetration of elements by means of rapid thermal ashing. Spectra of hair and their ashes are analysed. Sensitivity increase coefficients for heavy metals and some other chemical elements are calculated.